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Cell Counting Kit 8 product with CAT#CK04 from Dojindo Laboratories

Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 to produce a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. Since CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation is applicable.

As a well-known product, Cell Counting Kit-8 (CCK-8) Dojindo with CAT#CK04 has been widely used all around the world. It allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, WST or MTS.

CCK-8 Vendor Cat # Size
Cell Counting Kit 8 with Item # CK4 CK04 100/ 500/ 1000/ 3000/ 10000 tests
CCK-8 (# CK4) competitor from Abbkine  KTC011001 100/ 500/ 2000/ 10000  tests

Note: Each test is for one well of samples in 98 plates,which means 10 μl of CCK-8 solution for one test.

You may check size and price of CCK-8 with item # CK04 from their online store. Lead time of this product is calculated based on production, payment method, destination, and your shipping option.

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The handling time of Cell Counting Kit-8 is the shortest among its competitors. Only 15 minutes of handling time is needed for Cell Counting Kit-8, whereas longer handling time is required for both MTS and MTT assay. It only takes 3 simple steps to complete whole cell assay. And this product is for research use only.

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Preparing Cell Counting Kit-8 Solution ?

Cell Counting Kit-8 (CCK-8) solution in kit assign very convenient assays by utilizing highlywater-relaxed tetrazolium sailor, WST-8 to produce a water-dissolvable formazan Dyeupon decrease in the person of an electron porter, as shown in below.  WST-8 is educed by dehydrogenases in cells to give a yellowcolored roduct (formazan), which is soluble in the membrane civilization medium. The amount of the formazan dye generated by the nimbleness of dehydrogenases in cells is soon relative to the number of ignited cells.

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“The CCK-8 Kit has been optimized for use.” Said Brooke Kilyanek, Technical Manager of Abbkine Scientici. “If you want to buy WST-8 and 1-methoxy-PMS, and then mix the two regents  together to Cell counting kit-8 solution for cell proliferation assays yourselves. This is also certainly an option, but the best protocol to use is ultimately up to the researcher. “

Cell Counting Kit-8 is a one-deadman solution, and no mixing of components is required, which is totally differently from other commercial player in the market, such as WST-8 Kit. WST-8 Kit is normal made from two solution components. One component is an electron carrier for NADH-tetrazolium, which is 1-methoxy-PMS in most cases. And another component is WST-8 solution with proper reaction buffer.  While Cell Counting Kit-8 featured only one optimized solution component, which is nonradioactive, allot sensitive colorimetric assays for the purpose of the reckon of viable cells proliferation and cytotoxicity risk.

Since the CCK-8 solution is very stable and it has weak cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible. We highly suggest you purchase bulk size of Cell Counting Kit-8 if you’re willing to prepare CCK-8 solutions yourself. Since this will not only saving your time and cost, but also ensuring you reliability of cell research. Believe me, high quanlity branded CCK-8 allows you archieve colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays with high performance and high throughput.

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How to Choose right Cell Counting Kit-8 product

Choosing a cell viability or cytotoxicity assay from among the many different options available can be a challenging task. Picking the best assay format to suit particular needs requires an understanding of what each assay is measuring as an endpoint, of how the measurement correlates with cell viability, and of what the limitations of the assay chemistries are.

Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. CCK-8 highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allow sensitive colorimetric assays for the determination of the number of viable cells n cell proliferation and cytotoxicity assays.