Choosing a cell viability or cytotoxicity assay from among the many different options available can be a challenging task. Picking the best assay format to suit particular needs requires an understanding of what each assay is measuring as an endpoint, of how the measurement correlates with cell viability, and of what the limitations of the assay chemistries are.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. CCK-8 highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allow sensitive colorimetric assays for the determination of the number of viable cells n cell proliferation and cytotoxicity assays.