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Cell Counting Kit 8 product with CAT#CK04 from Dojindo Laboratories

Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 to produce a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. Since CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation is applicable.

As a well-known product, Cell Counting Kit-8 (CCK-8) Dojindo with CAT#CK04 has been widely used all around the world. It allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, WST or MTS.

CCK-8 Vendor Cat # Size
Cell Counting Kit 8 with Item # CK4 CK04 100/ 500/ 1000/ 3000/ 10000 tests
CCK-8 (# CK4) competitor from Abbkine  KTC011001 100/ 500/ 2000/ 10000  tests

Note: Each test is for one well of samples in 98 plates,which means 10 μl of CCK-8 solution for one test.

You may check size and price of CCK-8 with item # CK04 from their online store. Lead time of this product is calculated based on production, payment method, destination, and your shipping option.

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The handling time of Cell Counting Kit-8 is the shortest among its competitors. Only 15 minutes of handling time is needed for Cell Counting Kit-8, whereas longer handling time is required for both MTS and MTT assay. It only takes 3 simple steps to complete whole cell assay. And this product is for research use only.

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Preparing Cell Counting Kit-8 Solution ?

Cell Counting Kit-8 (CCK-8) solution in kit assign very convenient assays by utilizing highlywater-relaxed tetrazolium sailor, WST-8 to produce a water-dissolvable formazan Dyeupon decrease in the person of an electron porter, as shown in below.  WST-8 is educed by dehydrogenases in cells to give a yellowcolored roduct (formazan), which is soluble in the membrane civilization medium. The amount of the formazan dye generated by the nimbleness of dehydrogenases in cells is soon relative to the number of ignited cells.

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“The CCK-8 Kit has been optimized for use.” Said Brooke Kilyanek, Technical Manager of Abbkine Scientici. “If you want to buy WST-8 and 1-methoxy-PMS, and then mix the two regents  together to Cell counting kit-8 solution for cell proliferation assays yourselves. This is also certainly an option, but the best protocol to use is ultimately up to the researcher. “

Cell Counting Kit-8 is a one-deadman solution, and no mixing of components is required, which is totally differently from other commercial player in the market, such as WST-8 Kit. WST-8 Kit is normal made from two solution components. One component is an electron carrier for NADH-tetrazolium, which is 1-methoxy-PMS in most cases. And another component is WST-8 solution with proper reaction buffer.  While Cell Counting Kit-8 featured only one optimized solution component, which is nonradioactive, allot sensitive colorimetric assays for the purpose of the reckon of viable cells proliferation and cytotoxicity risk.

Since the CCK-8 solution is very stable and it has weak cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible. We highly suggest you purchase bulk size of Cell Counting Kit-8 if you’re willing to prepare CCK-8 solutions yourself. Since this will not only saving your time and cost, but also ensuring you reliability of cell research. Believe me, high quanlity branded CCK-8 allows you archieve colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays with high performance and high throughput.

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How to Choose right Cell Counting Kit-8 product

Choosing a cell viability or cytotoxicity assay from among the many different options available can be a challenging task. Picking the best assay format to suit particular needs requires an understanding of what each assay is measuring as an endpoint, of how the measurement correlates with cell viability, and of what the limitations of the assay chemistries are.

Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. CCK-8 highly water-soluble tetrazolium salt, WST-8, is reduced by dehydrogenase activities in cells to give a yellow-color formazan dye, which is soluble in the tissue culture media. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required. Cell Counting Kit-8, being nonradioactive, allow sensitive colorimetric assays for the determination of the number of viable cells n cell proliferation and cytotoxicity assays.

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Cell Counting Kit 8 price analysis

Cell Counting Kit-8 (CCK-8) is designed to detect cell number or cell prolifiration and toxicity without a solubilization process obtaining highly reproducible and accurate results. This kit is also compatible with high-throughput screening, thus reducing cost is one concern for researchers with large scale analysis.

Let’s take a look at 2017 Yr price of Cell Counting Kit-8 products in the market. Here we choose top 4 CCK-8 vendors as below for your quick review.

CCK-8 Supplier Price in 2017 Yr
Price of CCK-8 from Dojindo $42/100 Tests
$109/500 Tests
$188/1000 Tests
$397/3000 Tests
$1036/10000 Tests
Price of CCK-8 from Sigma (Now Millipore-Sigma)  $60.1/100 tests
$237.5/500 tests
$1190/3000 tests
Price of CCK-8 from Abbkine $20/100 tests
$64/500 tests
$205/2000 tests
$718/10000 tests
Price of CCK-8 from Abbkine $178/500 tests
$549/5*500 tests

Note: Each test is for one well of samples in 98 plates,which means 10 μl of CCK-8 solution for one test.

Donjindo is the well-known, which is also the fist supplier who launched CCK-8 products. They provide affordable price and good product quality with the most size option to researchers.

Sigma is another player in the marketing with great brand reputation and high quality, of course with the highest price. Due to aquisition of Sigma by Merck-Millipore, Merck-Millipore would suply this kit from 2016 in your region.

Abbkine’s CCK-8 featured the same quality of Donjindo while with more competitive price, which priced half compared with Dojindo, and 1/4 comapred with Sigma. Tests show that they perform the same result comparing to others.

In case of size option, both Donjindo and Abbkine provide more package sizes, especially in bulk 10000 tests. Also, special discount  of CCK-8 from Abbkine and Donjindo are available for larger bulk amounts larger than 10000 tests for these who process large scale development and production.

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Looking for Cell Counting Kit-8 protocol?

The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity. It is a ready-to-use solution that can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results.

Cell Counting Kit-8 can be utilized to do cell number counting, cell prolifiration or cytotoxicity assay based on your requirments.  So it make a tiny protocol deferences when you apply different assay, while the majority of  operation protocol are the same. However, Some equipment and materials should be prepared before assay: 1). Plate reader (450 nm filter), 2). 96-well plate, 3). 10 µl, 100-200 µl and multi-channel pipettes.

 

Looking for Cell Counting Kit-8 Protocol ?

More detailed manipulation procedures for Cell number counting and Cell proliferation and cytotoxicity sssays from Abbkine CCK-8 Kit are listed below for your references,

Cell Number Determination Protocol

1) Inoculate cell suspension (100 µl/well) in a 96-well plate. Also prepare wells that contain known numbers of viable cells (to create a calibration curve in step 5). Pre-incubate the plate in a humidified incubator (e.g., at 37 °C, 5% CO2).

2) Thaw the CCK-8 on the bench top or in a water bath at 37 °C if it is frozen. Note: It takes about 30 minutes on the bench top at 25 °C or 5 minutes in a water bath at 37 °C.

3) Add 10 µl of the CCK-8 solution to each well of the plate. Note: Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

4) Incubate the plate for 1-4 hours in the incubator.

5) Measure the absorbance at 450 nm using a microplate reader. Prepare a calibration curve using the data obtained from the wells that contain known numbers of viable cells.

Note: To measure the absorbance later, add 10 µl of 1% w/v SDS to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 48 hours.

Cell Proliferation and Cytotoxicity Assay Protocol

1) Dispense 100 µl of cell suspension (5000 cells/ well) in a 96-well plate.

2) Pre-incubate the plate for 24 hours in a humidified incubator (e.g., at 37 °C, 5% CO2).

3) Add 10 µl of various concentrations of toxicant into the culture media in the plate.

4) Incubate the plate for an appropriate length of time (e.g., 6, 12, 24 or 48 hours) in the incubator.

5) Thaw the CCK-8 on the bench top or in a water bath at 37 °C if it is frozen. Note: It takes about 30 minutes on the bench top at 25 °C or 5 minutes in the water bath at 37 °C.

6) Add 10 µl of CCK-8 solution to each well of the plate. Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

7) Incubate the plate for 1-4 hours in the incubator. Measure the absorbance at 450 nm using a microplate reader.

Note: To measure the absorbance later, add 10 µl of 1% w/v SDS to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 48 hours.

Be attention to conditions or chemicals that affect dehydrogenase activity in viable cells may cause discrepancy between the actual viable cell number and the cell number determined using the Abbkine CCK-8 assay.